The The Efectiveness of Jamblang (Syzygium cumini) Leaves Extract Inclusion Skim Milk-Egg Yolk Extender on Motility and Viability of Aceh Cattle Spermatozoa during Pre-Freezing and Post-Thawing
Keywords:
Spermatozoa of Aceh Cattle, Jamblang (Syzygium cumini) leaves extract, motility, viability, pre-freezing, post-thawing
Abstract
The process of semen freezing causes an increase in free radicals concentration which can damage spermatozoa. The addition of natural ingredients in semen diluent is expected to solve this challenges. One of the natural ingredients that can be used is jamblang (Syzygium cumini) leaves. The objective of the current study was to investigate the quality of spermatozoa in Aceh cattle which was added with jamblang leaves extract in skim milk- egg yolk extender during pre-freezing and post-thawing. This study applied Completely Randomized Design (CRD) with 5 treatments and 5 replications. The treatments consisted of J0 = skim milk-egg yolk; J1 = skim milk-egg yolk + jamblang leaves extract 0.2%; J2 = skim milk-egg yolk + jamblang leaves extract 0.4%; J3 = skim milk-egg yolk + jamblang leaves extract 0.6%; and J4 = skim milk-egg yolk + jamblang leaves extract 0.8%. The parameters observed in this study were the percentage of motility and viability of frozen semen of Aceh cattle. The data obtained were analyzed using Analysis of Variance (ANOVA) and if differences were found, then it would be continued with Duncan's Multiple Distance test. The results showed that the addition of jamblang leaves extract in egg yolk skim milk significantly affected the percentage of motility during pre-freezing and post-thawing, significantly affected spermatozoa viability during pre-freezing and significantly affected the spermatozoa viability during post-thawing. J3 treatment (jamblang leaves extract 0.6 gram/100 ml) it should be higher than the other treatment, where the percentage of motility at pre-freezing and post-thawing were 55.48% and 52.71%, respectively, and the percentage of viability during pre-freezing and post-thawing were 56.59% and 53.94%, respectively. It was concluded that the addition of jamblang leaves extract in the skim milk-egg yolk extender affected the percentage of spermatozoa motility and viability of Aceh cattle during pre-freezing and post-thawing.
References
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Dewi, A. S., Ondho, Y. S., & Kurnianto, E. (2012). Kualitas semen berdasarkaan umur pada sapi jantan jawa. Indian Vet J., 63, 909-912.
Ezzati, M., Shanehbandi, D., Hamdi, K., Rahbar, S., & Pashaiasl, M. (2020). Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview. Cell and Tissue Banking, 21, 1-15. https://doi.org/10.1007/s10561-019-09797-0
Feradis. (2009). Peranan antioksidan dalam pembekuan semen. Jurnal Peternakan, 6(2), 63-70. http://dx.doi.org/10.24014/jupet.v6i2.379
Ferramosca, A., & V. Zara. (2014). Bioenergetics of mammalian sperm capacitation. Review Article. Biomed Research International, 2014, 1-8. https://doi.org/10.1155/2014/902953
Fraser, L., & Strzezek, J. (2005). Effects of freezing-Thawing on DNA integrity of boar spermatozoa assessed by neutral comet assay. Reproduction in Domestic Animals, 40(6), 530-536. https://doi.org/10.1016/j.anireprosci.2006.06.003
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Gillan, L., Maxwell, W. M. C., & Evans, G. (2004). Preservation and evaluation of semen for artificial insemination. Reproduction, Fertility and Development, 16(4), 447-454. https://doi.org/10.1071/RD04034
Gowri, S. S., & Vasantha, K. (2010). Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) Leaves Extracts. PhamTech Res., 2(2), 1569-1573.
Hafez, E. S. E. (2004). Reproduction in farm animals. 7th edition. Lea and Febiger. Philadelphia.
Hanifi, H., Ihsan, M. N., & Susilawati, T. (2016). Pengaruh Lama Ekuilibrasi pada Proses Pembekuan terhadap Kualitas Semen Sapi Wagyu menggunakan Pengencer Andromed®. J. Ternak Tropika, 17(1), 31-41. https://doi.org/10.21776/ub.jtapro.2016.017.01.4
Holden, S. A., Fernandez-Fuertes, B., Murphy, C., Whelan, H., O’Gorman, A., Brennan, L., … S. Fair. (2017). Relationship between in vitro sperm functional assessments, seminal plasma composition, and field fertility after AI with either non-sorted or sex-sorted bull semen. Theriogenology, 87, 221-228. https://doi.org/10.1016/j.theriogenology.2016.08.024
Januskauskas, A., Johannison, A., & Rodriguez-Martinez. H. (2003). Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology, 60(4), 743-758. https://doi.org/10.1016/S0093-691X(03)00050-5
Khan, M. I. R., & Ijaz, A. (2008). Effects of osmotic pressure on motility, plasma membrane integrity and viability in fresh and frozen-thawed buffalo spermatozoa. Animal, 2(4), 548-553. https://doi.org/10.1017/S1751731108001596
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