Influence of Shade and Bed Types on Attaining Optimum Temperature for The Germination of Peach (Prunus Persica L. Batsch) Seeds at Holetta, Central Ethiopia
Abstract
Poor germination of peach seeds is the major limiting factor for rootstock production under the Holetta condition because of the seed dormancy problem. Stratification plays an important role in shortening the dormancy and increasing the germination of peach seeds. The study aimed to find easy stratification techniques for the germination of peach seeds using shade and different bed types. The experiment was carried out at the Holetta Agricultural Research Center in two successive years of 2017 and 2018. We got seeds used for this study from peach trees, which were well adapted for the Holetta area. It was arranged in a completely randomized design with five treatments of under tree shade on a flatbed, under tree shade in sunken bed, out of tree shade on a flatbed, out of tree shade in sunken bed and refrigerator as a standard check. They put all treatments except the refrigerator under the grass shade constructed 1 m3 wide. The treatments replicated four times, and each replication contained 200 seeds. The results showed that temperature is the most important factor affecting the germination percentage of the peach seeds. Seeds sown under the temperature close to 8 0C which is in the refrigerator found to have better germination percentage (47.1%) followed by the seeds sown in the grass shade under the tree shade both on sunken (35.8%) and flatbeds (34.9%). Peach seeds stratified in the refrigerator had good germination percentage whereas survival of the seedling was better at out of tree shade in sunken bed and out of tree shade on the flatbed. Even though the germination percentage was lower and the germination speed index was higher than the refrigerator. Seeds stratified at a temperature of above 17 OC experienced a poor germination percentage.
References
Afroze, F., & O’Reilly, C. (2016). Effects of seed moisture content, warm, chilling, and exogenous hormone treatments and germination temperature on the germination of blackthorn seeds. Plant Biosystems, Rev. Ceres, Viçosa, 60(2), 234-241. https://doi.org/10.1080/11263504.2016.1179693
Allen, P. S., Benech-Arnold, R. L., Batlla, D., & Bradford, K. J. (2007). Modeling of seed dormancy. In: Bradford KJ, Nonogaki H (eds), Seed development, dormancy and germination (pp 72–112). Blackwell Publishing, Oxford. https://doi.org/10. 1002/9780470988848
Al-Mudaris, M. (1998). Notes on various parameters recording the speed of seed germination. Der TropenlandWirt, 99, 147-154.
Americo W. J., Bruckner, C. H., Silva, J. O. C., Santos, C. E. M., Pimentel, L. D., & Mazaro, S. M. (2013). Chilling requirement for seed germination and phenological observations on peach cultivars. Rev. Ceres, Viçosa, 60(2), 234-241. https://doi.org/10.1590/S0034-737X2013000200012
Bentsink, L., & Koornneef, M. (2008). Seed dormancy and germination. The Arabidopsis Book/American Society of Plant Biologists. https://doi.org/10.1199/tab.0119
Bewley, J. D., Kent, J. B. , Henk, W. M., & Hiro, N. (2013). Seeds: Physiology of development, germination and dormancy, 3rd edition. Springer Science+Business Media, LLC. New York, USA. https://doi.org/10.1007/978-1-4614-4693-4
Biggs, R. H., & Langan, M. C. (1962). Effect of Temperature on Germination of Okinawa Peach Seeds. Fla. State Hort. Soc. Proc., 75, 379–81.
Chauhan, K. S., Biggs, R. H., & Sites, J. W. (1961). Temperature and dormancy of peach seeds. Proc. Fla. State Hort. Soc., (74), 367-371.
EIAR (Ethiopian Institute of Agricultural Research). (2017). Holetta Agricultural Research Center Progress Report. HARC, Holetta, Ethiopia.
Esechie, H. (1994). Interaction of salinity and temperature on the germination of sorghum. Journal of Agronomy and Crop Science, 172, 172-199.https://doi.org/10.1111/j.1439-037X.1994.tb00166.x
Finch-Savage, W. E. (2004). Models that describe germination behavior. In: Benech-Arnold R, Sanchez RA (eds), Handbook of Seed Physiology. Applications to agriculture (pp. 51-95). Food Products Press, New York. https://doi.org/10.1017/S0021859605235347
Frisby, J. W., & Seeley, S. D. (1993). Chilling of endo-dormant peach propagules: Seed germination and emergence. J. Amer. Soc. Hort. Sci., 118, 248-252. https://doi.org/10.21273/JASHS.118.2.248
Geneve, R. L, (2003). Impact of temperature on seed dormancy. HortScince, 38(3), 336-341. https://doi.org/10.21273/HORTSCI.38.3.336
Hartmann, H. T., Kester, D. E., Davies, F. T., & Geneve, R. L. (1997). Plant Propagation: Principles and practices. (6th ed). Prentice-Hall, USA. https://doi.org/10.1086/409642
Holmes, M. G., & McCartney, H. A. (1975). Shade light quality. In: Smith H (ed), Light and plant development (pp 446–476). Butterworths, London. https://doi.org/10.1002/9780470988893
Kester D. E., Raddi, P., & Assay, R. (1977). Correlation of chilling requirements of germination, blooming and leafing within and among seedling populations of almond. J. Amer. Soc. Hort. Sci., 102, 145-148.
Labouriau, L. G. (1983). A new line of research in the physiology of seed germination. Proceedings of the XXXIV National Botany Congress. SBB, Porto Alegre. Pp:11-50.
Maguire, J. D. (1962). Speed of germination-aid selection and evaluation for seedling emergence and vigor. Crop Science, 2, 176-177.
Martinez-Gomes, P., & Dicenta, F. (2001). Mechanisms of dormancy in seeds of peach (Prunus persica L. Batsch) cv. GF305. Scientia Horticulturae, (91), 51-58. https://doi.org/10.1016/S0304-4238(01)00235-7
Martins, A. S., Bianchi, V. J., Zanandrea, I., Spinelli, V. M., & Fachinello, J. C. (2014). Effects of seeds stratification on seedling emergence and initial development of peach rootstock. Científica (Jaboticabal), 42(2), 366-375.
Mehanna, H. T., Martin, G. C., & Nishijima, C. (1985). Effects of temperature, chemical treatments and endogenous hormone content on peach seed germination and subsequent seedling growth. Hort. Science, 27, 63-73. https://doi.org/10.1016/0304-4238(85)90056-1
Orchard, T. J (1977). Estimating the parameters of plant seedling emergence. Seed Science and Technology, 5, 61-69.
Perez-Gonzalez, S. (1997). Genotypic differentiation in temperature requirements for stratification in peach. Hort. Science, (32), 1064-1068. https://doi.org/10.21273/HORTSCI.32.6.1064
Pollock, B. M. (1962). Temperature control of physiological dwarfing in peach seedlings. Plant Physiology, 35, 190-197. https://doi.org/10.1104%2Fpp.37.2.190
Powell, L. E. (1987). The hormonal control of bud and seed dormancy in woody plants. In: Davies P. J. (eds), Plant Hormones and their Role in Plant Growth and Development (pp. 539-552). Springer, Dordrecht. https://doi.org/10.1007/978-94-009-3585-3_28
Rawat, J. M. S., Tomar, Y. K., & Vidyawati, R. (2010). Effect of stratification on seed germination and seedling performance of wild pomegranate. Journal of American Science, 6(5), 97-99. https://doi.org/10.7537/marsjas060510.15
Seeley, S. D., Ayanoglu, H., & Frisby, J. W. (1998). Peach seedling emergence and growth in response to isothermal and cycled stratification treatments reveal two dormancy components. J. Am. Soc. Hort. Sci., 123(5), 776–780. https://doi.org/10.21273/JASHS.123.5.776
Selim, H. H., Kilany, O. A., Abdel-Wahab, W., & Hassan, T. Y. (1998). Physiological studies on the propagation of Nemaguard peach seeds. Arab Universities Journal of Agricultural Sciences, 6, 249-266.
Sharma, H. C., & Singh, R. N. (1978). Effect of stratification temperature, stratification period and seed coat on seed germination of peach cultivar ‘Sharbati’. Scientia Horticulturae, 9, 47-53. https://doi.org/10.1016/0304-4238(78)90108-5
Tang, Y., Zhang, K., Zhang, Y., & Tao, J. (2019). Dormancy-breaking and germination requirements for seeds of Sorbus alnifolia (Siebold & Zucc. ) K. Koch (Rosaceae), a Mesic forest tree with high ornamental potential. Forest, 10, 319-320. https://doi.org/10.3390/f10040319
Thakur, B. (2015). Effect of growth regulator, scarification and thiourea on seed germination in peach (Prunus persica L. Batsch) rootstock Flordaguard. Int. Curr. Res. Aca., Rev., 3(5), 252-261.
Vahdati, K., Asadolah, A. A., Majid, R., Darab, H., & Charles, L. (2012). Mechanism of seed dormancy and its relationship to bud dormancy in Persian walnut. Environmental and Experimental Botany, 75, 74–82. https://doi.org/10.1016/j.envexpbot.2011.08.006
Wagner, J. A., Horst, B. C., Osmar, C. S. J., Eduardo, M. S. C., Duarte, P. L., & Sergio-Miguez, M. S. (2013). Chilling requirement for seed germination and phenological observations on peach cultivars. Revista Ceres, 60(2), 234-241. https://doi.org/10.1590/S0034-737X2013000200012
Webb, D. P., van Staden, J., & Wareing, P. F. (1973). Seed dormancy in Acer: changes in endogenous cytokinins, gibberellins and germination inhibitors during the breaking of dormancy in Acer saccharum 'Marsh'. J. Exp. Botany, 24(78), 105-116. https://doi. org/10. 1093/jxb/24. 1. 105
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